补硒排重金属（补硒排毒）

这项研究的目的是研究长期施用猪粪对通过三种粪肥N-PM处理从嘉兴长期田间试验场采样的表层土壤中重金属，抗生素和ARG积累的影响。 0 kg / ha / y / dw），L-PM（7720 kg / ha / y，dw）和H-PM（11,580 kg / ha / y，dw）。结果显示，最严重两年中所有粪便样品中均记录了锌和铜的金属污染，其含量超过了土壤质量标准。在这三种四环素中，金霉素是检出的主要抗生素，长期施用猪粪后，2013年检出的范围为3.04-98.03μg·kg-1，2014年检出的范围为28.67-344.74μg·kg-1。Q-PCR结果表明，核糖体保护蛋白基因（tetM，tetO，tetQ和tetW）低于大多数外排泵基因（tetA和tetG）。在这两年中，施肥的位点的tet和sul基因的丰度明显高于未施肥的位点。ARG的元基因组学分析显示，多药耐药基因的丰富性是最丰富的亚型，其次是氟喹诺酮，杆菌肽，磺酰胺和四环素。ARGs水平之间呈正相关；两年中土壤有机质，抗生素，铜，砷和锌的含量。这些结果可能揭示了长期施用肥料对表层土壤中ARGs发生和传播的影响的机理。在这两年中，施肥的位点的tet和sul基因的丰度明显高于未施肥的位点。

ARG的元基因组学分析显示，多药耐药基因的丰富性是最丰富的亚型，其次是氟喹诺酮，杆菌肽，磺酰胺和四环素。ARGs水平之间呈正相关；两年中土壤有机质，抗生素，铜，砷和锌的含量。这些结果可能揭示了长期施用肥料对表层土壤中ARGs发生和传播的影响的机理。在这两年中，施肥的位点的tet和sul基因的丰度明显高于未施肥的位点。ARG的元基因组学分析显示，多药耐药基因的丰富性是最丰富的亚型，其次是氟喹诺酮，杆菌肽，磺酰胺和四环素。ARGs水平之间呈正相关；两年中土壤有机质，抗生素，铜，砷和锌的含量。这些结果可能揭示了长期施用肥料对表层土壤中ARGs发生和传播的影响的机理。其次是氟喹诺酮，杆菌肽，磺酰胺和四环素。ARGs水平之间呈正相关；两年中土壤有机质，抗生素，铜，砷和锌的含量。这些结果可能揭示了长期施用肥料对表层土壤中ARGs发生和传播的影响的机理。其次是氟喹诺酮，杆菌肽，磺酰胺和四环素。ARGs水平之间呈正相关；两年中土壤有机质，抗生素，铜，砷和锌的含量。这些结果可能揭示了长期施用肥料对表层土壤中ARGs发生和传播的影响的机理。

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Increased occurrence of heavy metals, antibiotics and resistance genes in surface soil after long-term application of manure.

The purpose of this study was to investigate the impact of long-term application of pig manure on the accumulation of heavy metals, antibiotics and ARGs in surface soil sampled from the Jiaxing long-term field experimental site with three manure treatments, N-PM (0 kg/ha/y, dw), L-PM (7720 kg/ha/y, dw), and H-PM (11,580 kg/ha/y, dw), in 2013 and 2014。 The results showed that most serious metal pollution of Zn and Cu was recorded in all manured samples in both years, and their contents exceeded the soil quality standards。

Among the three tetracyclines, chlortetracycline was the predominant antibiotic detected with a range of 3。04-98。03 μg·kg-1 in 2013 and 28。67-344。74 μg·kg-1 in 2014 after long-term pig manure application。 Q-PCR results showed that the average accumulation of ribosomal protection protein genes (tetM, tetO, tetQ and tetW) was lower than most of the efflux pump genes (tetA and tetG)。 The abundance of tet and sul genes of those sites with manure application was significantly higher than that of sites without manure application in both years。

Metagenomics analysis of ARGs revealed that the abundance of multidrug resistance genes was the most abundant subtype, followed by fluoroquinolone, bacitracin, sulfonamide and tetracycline。 There was a positive correlation between the levels of ARGs; soil organic matter, antibiotics, Cu, As, and Zn levels in both years。 These results may shed light on the mechanism underlining the effects of long-term manure application on the occurrence and dissemination of ARGs in surface soil。